Trimethyl Lock: A Stable Chromogenic Substrate for Esterases
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چکیده
منابع مشابه
Trimethyl lock: a stable chromogenic substrate for esterases.
p-Nitrophenyl acetate is the most commonly used substrate for detecting the catalytic activity of esterases, including those that activate prodrugs in human cells. This substrate is unstable in aqueous solution, limiting its utility. Here, a stable chromogenic substrate for esterases is produced by the structural isolation of an acetyl ester and p-nitroaniline group using a trimethyl lock moiet...
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Fluorescent molecules are essential for basic research in the biological sciences and have numerous practical applications. Herein is described the synthesis and use of a new class of latent fluorophores based on a novel design element, the trimethyl lock, that confers distinct advantages over extant fluorophores and pro-fluorophores. A diacetyl version of the latent fluorophore is stable in a ...
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CENTA, a chromogenic cephalosporin, is readily hydrolyzed by β-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of β-lactamaseproducing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions. Nitrocefin and, to a les...
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Fluorescent molecules are indispensable tools in modern biochemical and biological research, being used as labels for biomolecules, indicators for ions, stains for organelles, and substrates for enzymes. The major targets of this last class are hydrolases that catalyze the removal of a masking moiety, thereby modulating fluorescence. A critical property of fluorogenic hydrolase substrates is th...
متن کاملMechanistic Studies of the Photoinduced Quinone Trimethyl Lock Decaging Process.
Mechanistic studies of a general reaction that decages a wide range of substrates on exposure to visible light are described. The reaction involves a photochemically initiated reduction of a quinone mediated by an appended thioether. After reduction, a trimethyl lock system incorporated into the quinone leads to thermal decaging. The reaction could be viewed as an electron-transfer initiated re...
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ژورنال
عنوان ژورنال: Molecules
سال: 2008
ISSN: 1420-3049
DOI: 10.3390/molecules13020204